Journal: Nature Communications

Article Title: RhoB controls coordination of adult angiogenesis and lymphangiogenesis following injury by regulating VEZF1-mediated transcription

doi: 10.1038/ncomms3824

Figure Lengend Snippet: ( a ) Whole-mount staining of blood vessels (BS-I lectin) in the retina of wt and RhoB −/− pups subjected to hyperoxia from P7 to P12, and then returned back to normoxia from P12 to P17 (OD, optic disc). Histological (H&E) analysis of wt and RhoB −/− retinas at P17. Quantification of nuclei number interior to ILM ( n =10 mice per genotype, mean±s.e.m., unpaired two-tailed Student’s t -test). ( b ) Confocal analysis following whole-mount staining of CD31 expression in the granulation tissue present 7 days after ear wounding in adult wt and RhoB −/− mice (6–8 weeks old). Quantification of CD31-positive staining in the neovasculature of the granulation tissue (delineated with white lines) ( n =6 mice per genotype, mean±s.e.m., unpaired two-tailed Student’s t -test). ( c ) Confocal analysis following whole-mount staining of CD31 and podoplanin expression in the granulation tissue present 7 days after wounding. Arrowheads highlight lymphatic vessels and denote areas of colocalization of CD31 and podoplanin in the merged image, thus confirming the identity of the lymphatic vessels. Scale bars: whole-mount staining=100 μm, H&E sections=50 μm ( a ), 200 μm ( b , c ).

Article Snippet: Subsequently, LVECs were separated from BVECs by using goat anti-mouse Dynabeads associated with Podoplanin antibody (Angiobio).

Techniques: Staining, Two Tailed Test, Expressing

Journal: Nature Communications

Article Title: RhoB controls coordination of adult angiogenesis and lymphangiogenesis following injury by regulating VEZF1-mediated transcription

doi: 10.1038/ncomms3824

Figure Lengend Snippet: ( a ) Intravital microlymphangiography by in vivo injection of FITC–dextran (MW 2,000 kDa) into lymphatics of ear skin 7 days after ear wounding in adult wt and RhoB −/− mice. Dextran-positive areas in isolated frames taken from the movies at early (2–10 s (s)), middle (30 s) and late (60 s) times following tracer injection were quantified ( n =7 mice per genotype, mean±s.e.m., unpaired two-tailed Student’s t -test). ( b ) Confocal analysis following in vivo FITC–dextran injection in lymphatics and whole-mount staining of podoplanin expression in the granulation tissue present 7 days after ear wounding in adult wt and RhoB −/− mice. Arrowhead and star respectively highlight dextran leakage from an abnormal lymphatic vessel and tracer accumulation in the adjacent tissue of RhoB −/− mice in the merged image. ( c ) Induction of DTH reactions in the ear skin of adult wt and RhoB −/− mice. Ear swelling is expressed as the increase (Δ) over the original ear thickness in μm. Ear thickness was measured daily for 11 days following challenge using a Mitutoyo caliper ( n =12 mice per genotype, mean±s.e.m., unpaired two-tailed Student’s t -test). ( d ) Intravital microlymphangiography by in vivo injection of FITC–dextran (MW 2,000 kDa) into lymphatics of ear skin 7 days after inflammation induced by oxazolone in adult wt and RhoB −/− mice. FITC–dextran-positive areas in isolated frames taken from movies at early (2–10 s (s)), middle (30 s) and late (60 s) times following tracer injection were quantified ( n =6 mice per genotype, mean±s.e.m., unpaired two-tailed Student’s t -test). Scale bars: 1 mm ( a , d ) and 100 μm ( b ).

Article Snippet: Subsequently, LVECs were separated from BVECs by using goat anti-mouse Dynabeads associated with Podoplanin antibody (Angiobio).

Techniques: In Vivo, Injection, Isolation, Two Tailed Test, Staining, Expressing

Journal: Fertility and sterility

Article Title: Trophoblast retrieval and isolation from the cervix (TRIC) for noninvasive prenatal screening at 5 to 20 weeks of gestation

doi: 10.1016/j.fertnstert.2014.04.008

Figure Lengend Snippet: Expression of β-hCG in cells isolated using anti-HLA-G coupled to magnetic nanoparticles. Bound (A–C and G–I) and the excluded nonbound (D–F) cells were labeled with anti-β-hCG (A–F) or nonimmune IgG (G–I) and examined by immunofluorescence microscopy. Images of secondary antibody conjugated to FITC (green) appear in the left panels and matching fields of nuclei counterstained with DAPI (blue) appear in the center panels. Overlays of the FITC and DAPI stain appear in the right panels. Size bar is 50 μm.

Article Snippet: Mouse anti-HLA-G antibody (5 mg) was incubated overnight at 4°C in 100 mL sterile PBS containing 20 mL of 250 nm magnetic nanoparticles conjugated to goat anti-mouse IgG (Clemente Associates).

Techniques: Expressing, Isolation, Labeling, Immunofluorescence, Microscopy, Staining

Journal:

Article Title: Leukocyte-Mediated Endothelial Cell Injury and Death in the Diabetic Retina

doi:

Figure Lengend Snippet: CD18 blockade prevents leukocyte adhesion and endothelial cell injury and death in diabetic retinal venules and capillaries. A: After treatment with anti-CD-18 F(ab′)2, diabetic retinal leukocyte adhesion was markedly decreased in the retinal venules and capillaries. B: Analysis with PI revealed a concomitant decrease in retinal endothelial cell injury and death; however, no change in arteriolar endothelial cell injury and death was observed.

Article Snippet: In separate experiments, diabetic rats received either 1 mg/kg of a mouse anti-rat CD18 F(ab′) 2 (WT.3) (Associates of Cape Cod Inc., Falmouth, MA) or a purified mouse anti-human non-immune F(ab′) 2 (Jackson ImmunoResearch Laboratories Inc., West Grove, PA).

Techniques:

Journal:

Article Title: Leukocyte-Mediated Endothelial Cell Injury and Death in the Diabetic Retina

doi:

Figure Lengend Snippet: Anti-CD18 surface staining of a static leukocyte within a vessel. Green fluorescence from the FITC-linked Concanavalin A (A) and red fluorescence from the anti-CD18 antibody (B) identify the leukocytes as being CD18-positive when the images are superimposed (C).

Article Snippet: In separate experiments, diabetic rats received either 1 mg/kg of a mouse anti-rat CD18 F(ab′) 2 (WT.3) (Associates of Cape Cod Inc., Falmouth, MA) or a purified mouse anti-human non-immune F(ab′) 2 (Jackson ImmunoResearch Laboratories Inc., West Grove, PA).

Techniques: Staining, Fluorescence